Coomassie Staining as Loading Control in Western Blot Analysis

作者: Charlotte Welinder , Lars Ekblad

DOI: 10.1021/PR1011476

关键词:

摘要: … Each membrane piece was wet in 99.5% ethanol for 5 s and then washed once in 0.5 mL water, 30 min, and four times in 0.5 mL 40% acetonitrile (ACN), 25 mmol/L NH 4 HCO 3 before …

参考文章(12)
Anil D’souza, R. Hal Scofield, Protein Stains to Detect Antigen on Membranes Methods of Molecular Biology. ,vol. 1314, pp. 433- 440 ,(2009) , 10.1007/978-1-59745-542-8_43
Ruth Menque Methogo, Geneviève Dufresne-Martin, Patrice Leclerc, Richard Leduc, Klaus Klarskov, Mass spectrometric peptide fingerprinting of proteins after Western blotting on polyvinylidene fluoride and enhanced chemiluminescence detection. Journal of Proteome Research. ,vol. 4, pp. 2216- 2224 ,(2005) , 10.1021/PR050014+
Jon L. Pryor, Wenhao Xu, David W. Hamilton, Immunodetection after complete destaining of Coomassie blue-stained proteins on immobilon-PVDF Analytical Biochemistry. ,vol. 202, pp. 100- 104 ,(1992) , 10.1016/0003-2697(92)90213-Q
Takashi Yamatodani, Lars Ekblad, Elisabeth Kjellén, Anders Johnsson, Hiroyuki Mineta, Johan Wennerberg, Epidermal growth factor receptor status and persistent activation of Akt and p44/42 MAPK pathways correlate with the effect of cetuximab in head and neck and colon cancer cell lines. Journal of Cancer Research and Clinical Oncology. ,vol. 135, pp. 395- 402 ,(2009) , 10.1007/S00432-008-0475-2
Yong Lin, Yan Li, Yi Liu, Wenjun Han, Quanze He, Jianglin Li, Ping Chen, Xianchun Wang, Songping Liang, Improvement of gel-separated protein identification by DMF-assisted digestion and peptide recovery after electroblotting. Electrophoresis. ,vol. 30, pp. 3626- 3635 ,(2009) , 10.1002/ELPS.200900070
Isabel Romero-Calvo, Borja Ocón, Patricia Martínez-Moya, María Dolores Suárez, Antonio Zarzuelo, Olga Martínez-Augustin, Fermín Sánchez de Medina, Reversible Ponceau staining as a loading control alternative to actin in Western blots. Analytical Biochemistry. ,vol. 401, pp. 318- 320 ,(2010) , 10.1016/J.AB.2010.02.036
Charlotte Welinder, Bo Jansson, Mårten Fernö, Håkan Olsson, Bo Baldetorp, Expression of Helix pomatia Lectin Binding Glycoproteins in Women with Breast Cancer in Relationship to Their Mood Group Phenotypes Journal of Proteome Research. ,vol. 8, pp. 782- 786 ,(2009) , 10.1021/PR800444B
Angela Dittmer, Jürgen Dittmer, Beta-actin is not a reliable loading control in Western blot analysis. Electrophoresis. ,vol. 27, pp. 2844- 2845 ,(2006) , 10.1002/ELPS.200500785
Jose L. Luque-Garcia, Ge Zhou, Daniel S. Spellman, Tung-Tien Sun, Thomas A. Neubert, Analysis of Electroblotted Proteins by Mass Spectrometry: Protein Identification after Western Blotting Molecular & Cellular Proteomics. ,vol. 7, pp. 308- 314 ,(2008) , 10.1074/MCP.M700415-MCP200