Time-resolved protein nanocrystallography using an X-ray free-electron laser
作者： Janos Hajdu , Christina Y. Hampton , Andreas Hartmann , Robert Hartmann , Stefan Hau-Riege
DOI: 10.1364/OE.20.002706
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摘要: We demonstrate the use of an X-ray free electron laser synchronized with optical pump to obtain diffraction snapshots from photoactivated states large membrane protein complexes in form nanocrystals flowing a liquid jet. Light-induced changes Photosystem I-Ferredoxin co-crystals were observed at time delays 5 10 µs after excitation. The result correlates microsecond kinetics transfer I ferredoxin. undocking process that follows leads rearrangements crystals will terminally lead disintegration crystals. describe experimental setup and first time-resolved femtosecond serial crystallography results irreversible photo-chemical reaction Linac Coherent Light Source. This technique opens door structural studies dynamics biological systems.
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