Greater diversity of HIV-1 quasispecies in HIV-infected individuals with active tuberculosis.

摘要: A continual increase in intrapatient HIV-1 heterogeneity is thought to contribute evasion of host immune response and eventual progression AIDS. Tuberculosis (TB) diagnosed both early late during the course disease may diversity quasispecies by activating increasing replication. The authors examined whether altered HIV-1-infected individuals with TB. Blood samples were obtained from 7 HIV-1- infected patients active TB (HIV/TB patients) 9 (HIV Kampala Uganda (CD4 counts 0-650 cells/mcl HIV loads 700-750000 ribonucleic acid copies/ml). C2-C3 region envelope gene (env) was amplified nested polymerase chain reaction lysed peripheral blood mononuclear cells each patient then subject sequencing clonal-quasispecies analysis heteroduplex tracking (HTA). HTA env deoxyribonucleic fragments showed increased HIV/TB compared group. Further sequence on 10 individual clones for significantly greater mutation frequencies than patients. An be associated a TB-mediated However diverse population as opposed tight clusters suggests possible dissemination lung-derived isolates TB-affected organ. (authors)