Quantitative analysis of high-resolution trypsin-Giemsa bands on human prometaphase chromosomes

摘要: We have constructed ideograms of human prometaphase chromosomes from synchronized and standard 72-h lymphocyte cultures. G banding was achieved by a trypsin-Giemsa (or Wright's stain) method. In addition to light (white) dark (black) bands, we distinguished three different shades grey. This distinction is essential for proper identification the increasing number bands displayed high-resolution chromosomes. The relative amount chromatin in each category staining intensity has been calculated expressed as ‘light value.’ represent maximal discernible with some consistency on chromosomes, i.e., 721 euchromatic 62 ‘variable’ heterochromatic or heteromorphic bands. are based measurements. On selected printed copies chromosome derived cells individuals, width band measured relation length respective arm. measurements per were averaged used construction ideograms. distance border between sub-bands centromere scale, positions 0 at 1.0 p terminus q terminus. numbering system follows Paris Conference (1971) recommendations.