Localization of sequences specifying messenger RNA to light-staining G-bands of human chromosomes.
作者： J�rge J. Yunis , Macus Tien Kuo , Grady F. Saunders
DOI: 10.1007/BF00288617
关键词:
摘要: Total cytoplasmic polyadenylated RNA was isolated from the human lymphocyte cell line Wil2 by oligo(dT)-cellulose chromatography. Tritiated complementary DNA (cDNA) transcribed and used as a probe for in situ hybridization to metaphase chromosomes. The majority of G-negative or lightly staining bands were found be preferential sites hybridization.
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