Sirt2 epigenetically down-regulates PDGFRα expression and promotes CG4 cell differentiation.

摘要: We have previously found that Sirt2 enhanced the outgrowth of cellular processes and MBP expression in CG4 cells, where is suppressed by transcription factor Nkx2.2. However, detailed mechanism facilitating oligodendroglial cell differentiation remained unclear. In present study, we observed partially translocated into nuclei when cells were induced to differentiate. was detected at CpG island PDGFRα promoter via ChIP assay during process rather than state growth. deacetylated protein(s) bound simultaneously appeared facilitate histone3 K27 tri-methylation, both which are suppressive signatures on gene activation. bisulfate assay, identified significantly DNA methylation compared with control. Consistently, overexpression down-regulated cells. The knock-down or over-expression repressed proliferation, but promoted proliferation. Taken together, while initiated a process, through epigenetic modification suppression expression. repression mediated transition processes, i.e. from proliferating progenitor post-mitotic