Involvement of CD9 and PDGFR in migration is evolutionarily conserved from Drosophila glia to human glioma.
作者： Astrid Jeibmann , Kathrin Halama , Hanna Theresa Witte , Su Na Kim , Kristin Eikmeier
DOI: 10.1007/S11060-015-1864-4
关键词:
摘要: Platelet-derived growth factor receptor (PDGFR) signaling plays an important role in the biology of malignant gliomas. To investigate mechanisms modulating PDGFR gliomagenesis, we employed a Drosophila glioma model and genetic screen to identify genes interacting with Pvr, fly homolog PDGFRs. Glial expression constitutively activated Pvr (λPvr) led glial over migration lethality at late larval stage. Among 3316 dsRNA strains crossed against tester strain, 128 shifted pupal stage, including tetraspanin 2A (tsp2A). In second step knockdown all tetraspanins was investigated. Of only tsp2A partially rescued Pvr-induced phenotype. Human CD9 (TSPAN29/MRP-1), close tsp2A, found be expressed cell lines A172 U343MG as well majority glioblastoma samples (16/22, 73 %). Furthermore, situ proximity ligation assay revealed association α β. cells, blocked PDGF-BB stimulated migration. conclusion, modulation by is evolutionarily conserved from glia human
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