Nonadhesive culture system as a model of rapid sphere formation with cancer stem cell properties.

摘要: Background Cancer stem cells (CSCs) play an important role in tumor initiation, progression, and metastasis are responsible for high therapeutic failure rates. Identification characterization of CSC crucial facilitating the monitoring, therapy, or prevention cancer. Great efforts have been paid to develop a more effective methodology. Nevertheless, ideal model research is still evolving. In this study, we created nonadhesive culture system enrich CSCs from human oral squamous cell carcinoma lines with sphere formation characterize their properties further. Methods A was designed generate spheres SAS OECM-1 lines. A subsequent investigation properties, including stemness, self-renewal, chemo- radioresistance vitro, as well initiation capacity vivo, also performed. Results Spheres were formed cost-effectively time-efficiently within 5 7 days. Moreover, proved that these expressed putative markers exhibited chemoradiotherapeutic resistance, addition tumor-initiating self-renewal capabilities. Conclusions Using system, successfully established rapid cost-effective exhibits characteristics can be used cancer research.