Microglia in invertebrate ganglia.

摘要: Abstract The results of this study lend strong support to the concept existence in insects and molluscs a distinctive class neuroglial cells comparable vertebrate microglia. The evidence presented is as valid that used reference separate status microglia--i.e., demonstration close structural functional relationship these with immune system. As vertebrates, excision ganglia from three invertebrate species (the Planorbarius corneus Mytilus edulis insect Leucophaea maderae) their maintenance incubation media led an exodus small accumulation culture dish. During process, they underwent conformational changes stellate rounded, then more or less ameboid, those indicative process activation animals' immunocytes. Functional characteristics which translocated microglia-like share immunocytes are motility, phagocytotic activity, adherence Furthermore, two have certain biochemical features common--e.g., presence cytokines (at least Planorbarius) corticotropin. An additional phenomenon particular interest for classification microglial elements response morphine. At 10(-6) M, drug decreases not only number emerging excised but also degree transformation "active" ameboid form. This dose-dependent naloxone-sensitive effect morphine on parallels activated same species. Corresponding demonstrating inhibitory mobilized frog Rana pipiens indicate between cell types under consideration exists vertebrates. Binding displacement experiments membrane homogenates well shown effects both mediated by special opiate receptor (mu 3).