Matrix metalloproteinase 2 activity decreases in human periodontal ligament fibroblast cultures submitted to simulated orthodontic force

摘要: Orthodontic force compresses the periodontal ligament promoting expression of pro-inflammatory mediators and matrix metalloproteinases responsible for tooth movement. The extent in time while cells are being treated increment amount mechanical stress caused by orthodontic is thought to regulate levels tissue. To study possible regulation activity 2, 3, 7, 9, 10 simulated force, human fibroblast cultures were centrifuged (141×g) 30, 60, 90, 120 min, simulating force. Cell viability, protein quantification, zymography evaluated at 24, 48, 72 h after centrifugation both cell lysates growth medium. 72-kDa metalloproteinase 2 was decreased 24 regardless duration 48 30 min only. Decrease total seen with no change viability. data seem indicate that regulates secreted 2. In addition, as a model may be used simple reliable method role played when submitted occurring during