Protein profiling of B-cell lymphomas using tissue biopsies: A potential tool for small samples in pathology

摘要: Non-Hodgkin's lymphoma comprises many related but distinct diseases and diagnosis classification is complex. Protein profiling of biopsies may be potential value for use in this the discovery novel markers. In study, we have optimized a method SELDI-TOF MS based protein frozen tissue sections, without dissection tumour cells. First compared chip surfaces lysis buffers. Also, determined minimal input using laser microscopy. Subsequently, analyzed profiles diffuse large B-cell (n=8), follicular (n=8) mantle cell (n=8). Benign, reactive lymph nodes (n=14) were used as reference group.CM10 surface combination with urea buffer an approximately 50,000 lymphocytes allowed detection differential peaks. Identification cases was reliably made supervised classification. Unsupervised clustering showed segregation into benign/indolent cluster predominantly formed by benign, more aggressive cases. conclusion, our protocol enables lysates derived from small histological samples subsequent differentially expressed proteins, need dissection. These results support further evaluation additional tool pathology.