Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus.

摘要: Abstract Mutations were targeted to the Hprt locus of mouse embryo-derived stem cells by using 22 different sequence replacement and insertion vectors. The targeting frequency was examined at two sites within as a function extent homology between vector target locus. also compared vectors prepared from isogenic nonisogenic DNA sources. With one exception, all showed same exponential dependence efficiency on This true regardless whether they or vectors, directed toward either locus, Vectors four five times more efficiently than did corresponding DNA. single case unexpectedly low involved could be attributed an unfavorable distribution heterology sequences present in endogenous gene.