Mutations in the gyrA and parC genes and in vitro activities of fluoroquinolones in 114 clinical isolates of Pseudomonas aeruginosa derived from urinary tract infections and their rapid detection by denaturing high-performance liquid chromatography
作者： Minori Matsumoto , Katsumi Shigemura , Toshiro Shirakawa , Yuzo Nakano , Hideaki Miyake
DOI: 10.1016/J.IJANTIMICAG.2012.06.021
关键词:
摘要: Fluoroquinolone (FQ) resistance in Pseudomonas aeruginosa has spread. The purpose of this study was to investigate the correlation between representative FQ, i.e. levofloxacin (LVX), and mutations gyrA parC genes P. clinical isolates from urine urinary tract infection patients their rapid detection by denaturing high-performance liquid chromatography (DHPLC). susceptibility LVX 114 measured quinolone resistance-determining regions (QRDRs) these were sequenced. DHPLC undertaken correlate distinctive chromatograms with DNA mutation patterns. Among tested, 22 (19.3%) resistant LVX. Six amino acid detected (Thr83Ile, Asp87Tyr Asp87Asn Ser87Leu, Ser87Trp Glu91Arg parC), existing alone or combination. There 10 kinds presence two more significantly correlated compared wild-type a single (P<0.0001). data identified number reproducibility distinguishable peak profile chromatogram. In conclusion, related aeruginosa. facilitated alleles, providing (5 min per sample), economical (96 samples run) reliable technique for characterising This system could forecast profile.
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