Association of overexpression of efflux pump genes with antibiotic resistance in Pseudomonas aeruginosa strains clinically isolated from urinary tract infection patients.
作者： Katsumi Shigemura , Kayo Osawa , Ayaka Kato , Issei Tokimatsu , Soichi Arakawa
DOI: 10.1038/JA.2015.34
关键词:
摘要: There are several mechanisms for antibiotic-resistant Pseudomonas aeruginosa. The purpose of this study is to investigate the association between expression efflux pump-coding genes and antibiotic resistance in P. aeruginosa causing urinary tract infections (UTIs). We extracted RNA from 105 clinical strains isolated UTI patients with full data on MICs assayed real-time quantitative reverse-transcription PCR. investigated gene expressions four nodulation cell division-type multi-drug pump systems (MexAB-OprM, MexCD-OprJ, MexEF-OprN MexXY(-OprA)) correlation nine antibiotics, risk factors resistance-related mexB, mexC, mexE mexY. Multivariate statistical demonstrated a significant relationship increased mexB or mexC complicated (Odds ratio=8.03, P<0.001 Odds ratio=8.86, P=0.032, respectively). also found levofloxacin (LVFX) ratio=4.48, P=0.035). In conclusion, leads LVFX UTI. These results contribute our knowledge system resistance.
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