Transfection of chicken embryo fibroblasts with Marek's disease virus DNA.

作者: Robin W. Morgan , John L. Cantello , Caroline H. McDermott

DOI: 10.2307/1591417

关键词:

摘要: Total DNA from Marek's disease virus (MDV)-infected chicken embryo fibroblasts was transfected into freshly plated secondary using calcium phosphate-mediated transfection. Transfection frequencies were dose-dependent and non-linear. The maximum transfection of nine MDV preparations 8-25 micrograms total ranged 45 to 898 plaques per phosphate/DNA precipitate. Approximately 100-200 60-mm tissue-culture dish 1-5 MDV-infected typically obtained. most efficient when the pH HEPES buffer 7.0, no additional carrier added precipitates, cultures exposed for 3 minutes 15% buffered glycerol 4 hours after addition precipitates.

参考文章(34)
Maotani K, Kato S, Ikuta K, Honma H, Ueda S, Hirai K, Monoclonal antibodies specific to and cross-reactive with Marek's disease virus and herpesvirus of turkeys. Biken's Journal. ,vol. 25, pp. 171- 175 ,(1982)
L. J. N. Ross, Molecular Biology of the Virus Marek’s Disease. pp. 113- 150 ,(1985) , 10.1007/978-1-4613-2613-7_5
E. Neumann, M. Schaefer-Ridder, Y. Wang, P.H. Hofschneider, Gene transfer into mouse lyoma cells by electroporation in high electric fields. The EMBO Journal. ,vol. 1, pp. 841- 845 ,(1982) , 10.1002/J.1460-2075.1982.TB01257.X
K. Nazerian, R. L. Witter, Cell-free transmission and in vivo replication of Marek's disease virus. Journal of Virology. ,vol. 5, pp. 388- 397 ,(1970) , 10.1128/JVI.5.3.388-397.1970
K Fukuchi, M Sudo, Y S Lee, A Tanaka, M Nonoyama, Structure of Marek's disease virus DNA: detailed restriction enzyme map. Journal of Virology. ,vol. 51, pp. 102- 109 ,(1984) , 10.1128/JVI.51.1.102-109.1984
R J Isfort, H J Kung, L F Velicer, Identification of the gene encoding Marek's disease herpesvirus A antigen. Journal of Virology. ,vol. 61, pp. 2614- 2620 ,(1987) , 10.1128/JVI.61.8.2614-2620.1987
S Kawai, M Nishizawa, New procedure for DNA transfection with polycation and dimethyl sulfoxide. Molecular and Cellular Biology. ,vol. 4, pp. 1172- 1174 ,(1984) , 10.1128/MCB.4.6.1172
R L Witter, X Liu, L F Lee, Monoclonal antibodies with specificity for three different serotypes of Marek's disease viruses in chickens. Journal of Immunology. ,vol. 130, pp. 1003- 1006 ,(1983)
F.L. Graham, A.J. van der Eb, A new technique for the assay of infectivity of human adenovirus 5 DNA Virology. ,vol. 52, pp. 456- 467 ,(1973) , 10.1016/0042-6822(73)90341-3
W. Tao, J. Wilkinson, E. J. Stanbridge, M. W. Berns, Direct gene transfer into human cultured cells facilitated by laser micropuncture of the cell membrane. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 84, pp. 4180- 4184 ,(1987) , 10.1073/PNAS.84.12.4180