Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification.
作者： E. Gómez , A. Rodríguez , M. Muñoz , J.N. Caamaño , C.O. Hidalgo
DOI: 10.1016/J.THERIOGENOLOGY.2007.12.015
关键词:
摘要: Abstract The aim of this study was to examine the effects co-culture with Vero cells during in vitro maturation (IVM) and three culture media, B2+5% fetal calf serum (FCS) on cells, synthetic oviduct fluid (SOF)+5% FCS, SOF+20gL −1 bovine albumin (BSA), developmental competence embryos their ability survive vitrification/warming. We also tested effect morphological quality age embryo its sensitivity vitrification. IVM system neither affects development up Day 7 nor survival rates after SOF+FCS cells+B2 allowed obtaining more 6 blastocysts, a higher % blastocysts vitrified than SOF+BSA. Contrarily, 8, were SOF+BSA SOF+FCS. Blastocysts affected vitrification/warming, showed 8 counterparts. produced or survived at those 24 48h warming. Embryo BSA allows hatching vitrification/warming obtained B2 FCS. Moreover, provides from comparable cells. Further studies, including transfer recipients, are needed clarify factors affecting freezability embryos.
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sci-hub.se 参考文章(50)
Jan Dijkstra, John Michael Forbes, James France, Quantitative Aspects of Ruminant Digestion and Metabolism ,(2005)
F.R. Dunshea, D.W. Pethick, G.S. Harper, Fat metabolism and turnover Pethick, D.W. <https://researchrepository.murdoch.edu.au/view/author/Pethick, David.html>ORCID: 0000-0002-3255-7677 <http://orcid.org/0000-0002-3255-7677>, Harper, G.S. and Dunshea, F.R. <https://researchrepository.murdoch.edu.au/view/author/Dunshea, Frank.html> (2005) Fat metabolism and turnover. In: Dijkstra, J. and Forbes, J.M., (eds.) Quantitative Aspects of Ruminant Digestion and Metabolism. CAB International, Wallingford, Oxon, pp. 345-371.. pp. 291- 311 ,(2005)
Ian R. Gordon, Laboratory Production of Cattle Embryos ,(1994)
Carlos O Hidalgo Ordóñez, Itziar Fernández, Paloma Duque, Nieves Facal, Elena Díaz, JM Prendes, Juan Menéndez Fernández, Enrique Gómez, Lupicinio Prieto, Carmen Díez Fernández, Primeros terneros producidos in vitro tras punción ecoguiada de folículos ováricos Archivos De Zootecnia. ,vol. 51, pp. 411- 422 ,(2002)
Y. Fukuda, M. Ichikawa, K. Naito, Y. Toyoda, Birth of normal calves resulting from bovine oocytes matured, fertilized, and cultured with cumulus cells in vitro up to the blastocyst stage. Biology of Reproduction. ,vol. 42, pp. 114- 119 ,(1990) , 10.1095/BIOLREPROD42.1.114
Hiroyuki Abe, Shoko Yamashita, Takeshi Satoh, Hiroyoshi Hoshi, Accumulation of cytoplasmic lipid droplets in bovine embryos and cryotolerance of embryos developed in different culture systems using serum-free or serum-containing media Molecular Reproduction and Development. ,vol. 61, pp. 57- 66 ,(2002) , 10.1002/MRD.1131
JK Thibodeaux, MW Myers, LL Goodeaux, Y Menezo, JD Roussel, JR Broussard, RA Godke, Evaluating an in vitro culture system of bovine uterine and oviduct epithelial cells for subsequent embryo co-culture. Reproduction, Fertility and Development. ,vol. 4, pp. 573- 583 ,(1992) , 10.1071/RD9920573
S. Saha, R. Rajamahendran, A. Boediono, C. Sumantri, T. Suzuki, Viability of bovine blastocysts obtained after 7, 8 or 9 days of culture in vitro following vitrification and one-step rehydration Theriogenology. ,vol. 46, pp. 331- 343 ,(1996) , 10.1016/0093-691X(96)00189-6
Kei IMAI, Satoko MATOBA, Osamu DOCHI, Itsuo SHIMOHIRA, Different factors affect developmental competence and cryotolerance in in vitro produced bovine embryo. Journal of Veterinary Medical Science. ,vol. 64, pp. 887- 891 ,(2002) , 10.1292/JVMS.64.887
P. Holm, P.J. Booth, M.H. Schmidt, T. Greve, H. Callesen, High bovine blastocyst development in a static in vitro production system using SOFaa medium supplemented with sodium citrate and myo-inositol with or without serum-proteins. Theriogenology. ,vol. 52, pp. 683- 700 ,(1999) , 10.1016/S0093-691X(99)00162-4