Different factors affect developmental competence and cryotolerance in in vitro produced bovine embryo.
作者： Kei IMAI , Satoko MATOBA , Osamu DOCHI , Itsuo SHIMOHIRA
DOI: 10.1292/JVMS.64.887
关键词:
摘要: The present study was conducted to examine the effects of culture systems and media on developmental competence freezability bovine embryos obtained by in vitro matured fertilized (IVM-IVF) oocytes. No significant difference observed proportions oocytes developed blastocysts, speed at which reached blastocyst stage number cells, when IVM-IVF were cultured CR1aa with or without cumulus cells. Nevertheless, more either cells seen reach much sooner than those TCM199 (P<0.05). proportion day 7 significantly higher TCM199. Viability after frozen-thawed blastocysts vitro, a percentage hatched These results indicate that superior for potential during regardless co-culture But inferior
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