Transplacental transmission of Toxoplasma gondii in reinfected pregnant female canines

摘要: Twelve pregnant female canines, naturally infected with Toxoplasma gondii, were reinfected T. gondii: three (GI) received tachyzoites subcutaneously (1.0 × 107), (GII) orally inoculated oocysts (1.5 104), and six (GIII) kept as a nonreinfected control group. All the canines (GI GII) miscarried or presented fetal death, while only one GIII stillborn in litter of four pups (P < 0.01). Fever, lymphoadenopathy, miscarriage, death main clinical alterations observed. The highest serological titers detected through indirect fluorescence antibody test (IFAT) 1,024 4,096 (GII). In group III, ranged between 64 256. By bioassays mice, gondii was isolated 17 organs adult 11 group, 20 neonates. Positive immunostaining cysts and/or observed 26 canine tissues (14 from GI GII ten GIII). agent by immunohistochemistry encephalon neonate spinal cord stillborn, thus, confirming that fetuses, provoking miscarriages, even bitches primoinfection.