Selective Killing of AFP-Positive Hepatocellular Carcinoma Cells by Adeno-Associated Virus Transfer of the Herpes Simplex Virus Thymidine Kinase Gene
作者： Hua Su , Judy C. Chang , Shan Mei Xu , Yuet Wai Kan
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摘要: ABSTRACT The use of viral thymidine kinase (TK) gene coupled with the administration ganciclovir to render cancer cell death has been studied extensively. Many these experiments utilized retrovirus transfer TK under control a nonspecific promoter. Because expression may cause proliferating cells, other than we explored liver-specific promoter and tumor-specific AFP enhancer achieve regulated for treatment hepatocellular carcinoma. We also used adeno-associated virus (AAV) as vector delivery because this is not associated any pathological consequences in humans. it can infect nondividing S-phase AAV genes into noncycling tumor cells. A recombinant was constructed include selectable marker neoR herpes simplex (HSV)-TK driven by human albumin T...
liebertonline.com参考文章(17)
ADRIAN M. DI BISCEGLIE, Hepatocellular Carcinoma Annals of Internal Medicine. ,vol. 108, pp. 390- 401 ,(1988) , 10.7326/0003-4819-108-3-390
K. Watanabe, A. Saito, T. Tamaoki, Cell-specific enhancer activity in a far upstream region of the human alpha-fetoprotein gene. Journal of Biological Chemistry. ,vol. 262, pp. 4812- 4818 ,(1987) , 10.1016/S0021-9258(18)61269-4
Kirk R. Thomas, Mario R. Capecchi, Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells Cell. ,vol. 51, pp. 503- 512 ,(1987) , 10.1016/0092-8674(87)90646-5
Cynthia A. Richards, Brian E. Huber, Generation of a Transgenic Model for Retrovirus-Mediated Gene Therapy for Hepatocellular Carcinoma Is Thwarted by the Lack of Transgene Expression Human Gene Therapy. ,vol. 4, pp. 143- 150 ,(1993) , 10.1089/HUM.1993.4.2-143
Mulligan RC Tybulewicz VL, Crawford CE, Jackson PK, Bronson RT, None, Neonatal lethality and lymphopenia in mice with a homozygous disruption of the c-abl proto-oncogene Cell. ,vol. 65, pp. 1153- 1163 ,(1991) , 10.1016/0092-8674(91)90011-M
K. Culver, Z Ram, S Wallbridge, H Ishii, E. Oldfield, R. Blaese, In vivo gene transfer with retroviral vector-producer cells for treatment of experimental brain tumors Science. ,vol. 256, pp. 1550- 1552 ,(1992) , 10.1126/SCIENCE.1317968
T R Flotte, S A Afione, P L Zeitlin, Adeno-associated virus vector gene expression occurs in nondividing cells in the absence of vector DNA integration. American Journal of Respiratory Cell and Molecular Biology. ,vol. 11, pp. 517- 521 ,(1994) , 10.1165/AJRCMB.11.5.7946381
S A Camper, S M Tilghman, Postnatal repression of the alpha-fetoprotein gene is enhancer independent. Genes & Development. ,vol. 3, pp. 537- 546 ,(1989) , 10.1101/GAD.3.4.537
R. A. Heyman, E. Borrelli, J. Lesley, D. Anderson, D. D. Richman, S. M. Baird, R. Hyman, R. M. Evans, Thymidine kinase obliteration: creation of transgenic mice with controlled immune deficiency. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 2698- 2702 ,(1989) , 10.1073/PNAS.86.8.2698
Y. Takamiya, M. P. Short, Z. D. Ezzeddine, F. L. Moolten, X. O. Breakefield, R. L. Martuza, Gene therapy of maliganant brain tumors: A rat glioma line bearing the herpes simplex virus type 1‐thymidine kinase gene and wild type retrovirus kills other tumor cells Journal of Neuroscience Research. ,vol. 33, pp. 493- 503 ,(1992) , 10.1002/JNR.490330316