Enhanced responsiveness to parathyroid hormone and induction of functional differentiation of cultured rabbit costal chondrocytes by a pulsed electromagnetic field.

摘要: Abstract Pulsed electromagnetic fields promote healing of delayed united and ununited fractures by triggering a series events in fibrocartilage. We examined the effects pulsed field (recurrent bursts, 15.4 Hz, shorter pulses an average 2 gauss) on rabbit costal chondrocytes culture. A slightly reduced intracellular cyclic adenosine 3′,5′-monophosphate (cAMP) level However, it significantly enhanced cAMP accumulation response to parathyroid hormone (PTH) 140% that induced PTH its absence, while did not affect prostaglandin E 1 or I . The effect became evident after exposure cultures for 48 h, was dependent upon strength. is followed induction ornithine decarboxylase, good marker differentiated chondrocytes, treatment 4 h. Consistent with accumulation, decarboxylase activity also increased 170% cells exposed field. Furthermore, stimulation glycosaminoglycan synthesis, phenotype, Thus, PTH. These findings show resulted functional differentiation cells.