Gradient Chitosan Hydrogels Modified with Graphene Derivatives and Hydroxyapatite: Physiochemical Properties and Initial Cytocompatibility Evaluation.

摘要: In this study, we investigated preparation of gradient chitosan-matrix hydrogels through a novel freezing–gelling–thawing method. The influence three types graphene family materials (GFM), i.e., oxide (GO), reduced (rGO), and poly(ethylene glycol) grafted (GO-PEG), as well hydroxyapatite (HAp) on the physicochemical biological properties composite was examined in view their potential applicability tissue engineering scaffolds. substrates hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, diffractometry, infrared digital scanning electron microscopy, rheological mechanical analysis, vitro chemical stability bioactivity assays, initial cytocompatibility evaluation with human umbilical cord Wharton’s jelly mesenchymal stem cells (hUC-MSCs). We followed green-chemistry approach avoided toxic cross-linking agents, using instead specific interactions our polymer matrix tannic acid, non-toxic physical cross-linker, derivatives. It shown that most promising are modified GO-PEG HAp.