Hydrogel membranes based on genipin-cross-linked chitosan blends for corneal epithelium tissue engineering
作者： Maria Grolik , Krzysztof Szczubiałka , Bogumił Wowra , Dariusz Dobrowolski , Bogusława Orzechowska-Wylęgała
DOI: 10.1007/S10856-012-4666-7
关键词: Materials science 、 Corneal epithelium 、 Polymer chemistry 、 Chitosan 、 Tissue engineering 、 Epithelium 、 Membrane 、 Cell culture 、 Genipin 、 Biophysics 、 Self-healing hydrogels
摘要: Novel polymeric hydrogel scaffolds for corneal epithelium cell culturing based on blends of chitosan with some other biopolymers such as hydroxypropylcellulose, collagen and elastin crosslinked genipin, a natural substance, were prepared. Physicochemical biomechanical properties these materials determined. The in vitro culture experiments cells have indicated that membrane prepared from chitosan–collagen blend (Ch–Col) provided the regular stratified growth cells, good surface covering increased number layers. Ch–Col membranes are therefore most promising material among those studied. performance is comparable amniotic which currently recommended clinical applications.
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