Characterization of a β-glucosidase from Paenibacillus species and its application for succinic acid production from sugarcane bagasse hydrolysate.
作者： Weiliang Dong , Menglei Xue , Yue Zhang , Fengxue Xin , Ce Wei
DOI: 10.1016/J.BIORTECH.2017.05.141
关键词:
摘要: In this study, a β-glucosidase from Paenibacillus sp. M1 was expressed in E. coli BL21(DE3), purified and characterized. The specific activity of BglA 137.64U·mg-1 protein with optimal temperature pH 50°C 6.0. Furthermore, shows excellent adaption to various environmental factors such as temperature, metal ions. Engineered Suc260 further reconstructed by overexpressing the for achieving direct cellobiose utilization, which could efficiently utilize pretreated sugarcane bagasses hydrolysate (SBH) consisting 25.30g·L-1 cellobiose, 9.70g·L-1 glucose, 5.90g·L-1 arabinose 7.10g·L-1 xylose. As result, 26.50g·L-1 24.30g·L-1 succinic acid were produced strain Suc260(pTbglA) SBH corresponding yields 88.30% 89.20% using dual-phase fermentation, respectively. This study indicated that incomplete enzymatic SCB will be potential feedstock production.
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