Succinate production by metabolically engineered Escherichia coli using sugarcane bagasse hydrolysate as the carbon source.

摘要: Abstract Efficient biosynthesis of succinate from a renewable biomass resource by engineered Escherichia coli is reported in this paper. Fermentation sugarcane bagasse hydrolysate E. BA204, pflB , ldhA and ppc deletion strain overexpressing the ATP-forming phosphoenolpyruvate carboxykinase Bacillus subtilis 168, produced final concentration 15.85 g L −1 with high yield 0.89 g L total sugar under anaerobic conditions. During dual-phase fermentations, initial aerobic growth facilitated subsequent production, 18.88 g L 0.96 g g after 24 h fermentation. The demonstrated great potential application as feedstock for economical production using metabolically .