Separation of protein charge variants with induced pH gradients using anion exchange chromatographic columns.
作者： Timothy M. Pabst , Giorgio Carta , Natrajan Ramasubramanyan , Alan K. Hunter , Paul Mensah
DOI: 10.1002/BTPR.53
关键词:
摘要: … This work concerns the chromatographic separation of protein charge variants using pH gradients generated by step changes in buffer composition with weak base anion exchange …
sci-hub.se 参考文章(46)
Melissa Perkins, Rolf Theiler, Susan Lunte, Margit Jeschke, Determination of the origin of charge heterogeneity in a murine monoclonal antibody. Pharmaceutical Research. ,vol. 17, pp. 1110- 1117 ,(2000) , 10.1023/A:1026461830617
Xuezhen Kang, Ronald C Bates, Douglas D Frey, High-performance chromatofocusing using linear and concave pH gradients formed with simple buffer mixtures. II. Separation of proteins. Journal of Chromatography A. ,vol. 890, pp. 37- 43 ,(2000) , 10.1016/S0021-9673(00)00573-2
MN Fukuda, H Sasaki, L Lopez, M Fukuda, Survival of recombinant erythropoietin in the circulation: the role of carbohydrates. Blood. ,vol. 73, pp. 84- 89 ,(1989) , 10.1182/BLOOD.V73.1.84.BLOODJOURNAL73184
Shuichi Yamamoto, Kazahiro Nakanishi, Ryuichi Matsuno, Ion-Exchange Chromatography of Proteins ,(1988)
Rolf G Werner, Kristina Kopp, Michael Schlueter, Glycosylation of therapeutic proteins in different production systems. Acta Paediatrica. ,vol. 96, pp. 17- 22 ,(2007) , 10.1111/J.1651-2227.2007.00199.X
Douglas M. Ruthven, Principles of Adsorption and Adsorption Processes ,(1984)
L.A.A.E. Sluyterman, O. Elgersma, Chromatofocusing: Isoelectric focusing on ion-exchange columns Journal of Chromatography A. ,vol. 150, pp. 17- 30 ,(1978) , 10.1016/S0021-9673(01)92092-8
Clayton A. Brooks, Steven M. Cramer, Steric mass‐action ion exchange: Displacement profiles and induced salt gradients Aiche Journal. ,vol. 38, pp. 1969- 1978 ,(1992) , 10.1002/AIC.690381212
Reed J. Harris, Bruce Kabakoff, Frank D. Macchi, Felicity J. Shen, May Kwong, James D. Andya, Steven J. Shire, Nancy Bjork, Klara Totpal, Anthony B. Chen, Identification of multiple sources of charge heterogeneity in a recombinant antibody. Journal of Chromatography B: Biomedical Sciences and Applications. ,vol. 752, pp. 233- 245 ,(2001) , 10.1016/S0378-4347(00)00548-X
John C. Strong, Douglas D. Frey, Experimental and numerical studies of the chromatofocusing of dilute proteins using retained pH gradients formed on a strong-base anion-exchange column Journal of Chromatography A. ,vol. 769, pp. 129- 143 ,(1997) , 10.1016/S0021-9673(96)01049-7