Steric mass‐action ion exchange: Displacement profiles and induced salt gradients

摘要: The study of nonlinear competitive equilibrium is fundamental importance in understanding the behavior proteins preparative ion-exchange chromatographic separations. In this work we present a steric mass-action (SMA) formalism, which explicitly accounts for hindrance salt counterions upon protein binding multicomponent equilibria. An analytical solution has been derived calculation isotachic effluent profiles displaced and induced gradients under ideal conditions. A stability analysis employed to establish order feed components displacement train. Theoretical predictions are compared experimental results separation by cation-exchange chromatography. These demonstrate efficacy SMA formalism predicting complex systems. Furthermore, with enables rapid methods development optimization