Decrease in the number of lower affinity (type II) nerve growth factor receptors on embryonic sensory neurons does not affect fiber outgrowth.
作者： Robert W. Stach , Barbara J. Wagner
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摘要: Nerve growth factor binds to two different specific receptors on responsive cells. The relationship of these is not fully understood at this time. We have studied the binding labeled NGF a strain white leghorn chicken embryo dorsal root ganglionic equilibrium dissociation constants for sites (KdI = 4.1 +/- 1.8 x 10(-11) M, KdII 1.0 0.8 10(-9) M) are identical those obtained previously. Also, number type I per cell (3.8 1.3 10(3)) same as that previously determined. However, II (1.9 10(4)) significantly than This 2.5-fold decrease in does affect concentration needed obtain maximal fiber outgrowth from explanted sensory ganglia. rate association (1.2 0.2 10(7) M-1 sec-1 22 degrees C) with neurons chickens obtained. its was also determined 4 C. constant (2.1 1.1 10(6) sec-1) along and 37 C were used determine an activation energy receptors. (16.2 kcal/mole) suggests diffusion-controlled process.
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