Fast and slow nerve growth factor binding sites in human neuroblastoma and rat pheochromocytoma cell lines: relationship of sites to each other and to neurite formation
作者： KH Sonnenfeld , DN Ishii
DOI: 10.1523/JNEUROSCI.05-07-01717.1985
关键词: Biophysics 、 Nerve growth factor 、 Trypsin 、 Binding site 、 Neuroblastoma 、 Neurite 、 Endocrinology 、 Nerve growth factor binding 、 Liaison 、 Internal medicine 、 Chemistry 、 Cell culture
摘要: We studied (a) the distribution and properties of fast slow 125I- nerve growth factor (125I-NGF) binding sites in cultured human neuroblastoma (NB) cell lines that were categorized as responsive (N+) or unresponsive (N-) to NGF by neurite outgrowth, (b) whether mediate actions NGF, (c) NGF-mediated conversion occurs NB pheochromocytoma PC 12 cells. In SH-SY5Y cells, trypsin resistant was high affinity. Loss had a half-time 25 30 min at 37 degrees C very 4 C. contrast, sensitive lower affinity; its dissociation less than 1 The association rate constants both about 0.8 1.2 X 10(7) M-1 sec-1. Some cells sites. N+ LA-N-5 only Despite virtual elimination MC-IXC remaining could still efficiently bind 125I-NGF. These observations showed are not required for site binding, other demonstrated some 125I-NGF initially bound directly transferred conversion. PC12 2 kinetics consistent with simple bimolecular reactions combined evidence did support hypothesis
sci-hub.se 参考文章(43)
P. Cohen, A. Sutter, G. Landreth, A. Zimmermann, E.M. Shooter, Oxidation of tryptophan-21 alters the biological activity and receptor binding characteristics of mouse nerve growth factor. Journal of Biological Chemistry. ,vol. 255, pp. 2949- 2954 ,(1980) , 10.1016/S0021-9258(19)85833-7
June L. Biedler, Barbara A. Spengler, Lawrence Helson, Morphology and Growth, Tumorigenicity, and Cytogenetics of Human Neuroblastoma Cells in Continuous Culture Cancer Research. ,vol. 33, pp. 2643- 2652 ,(1973)
P. Calissano, M.L. Shelanski, Interaction of nerve growth factor with pheochromocytoma cells. Evidence for tight binding and sequestration. Neuroscience. ,vol. 5, pp. 1033- 1039 ,(1980) , 10.1016/0306-4522(80)90184-0
L. A. Greene, A. S. Tischler, Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 73, pp. 2424- 2428 ,(1976) , 10.1073/PNAS.73.7.2424
R D Vale, E M Shooter, Alteration of binding properties and cytoskeletal attachment of nerve growth factor receptors in PC12 cells by wheat germ agglutinin. Journal of Cell Biology. ,vol. 94, pp. 710- 717 ,(1982) , 10.1083/JCB.94.3.710
Esperanza Recio-Pinto, Douglas N. Ishii, Effects of insulin, insulin-like growth factor-II and nerve growth factor on neurite outgrowth in cultured human neuroblastoma cells Brain Research. ,vol. 302, pp. 323- 334 ,(1984) , 10.1016/0006-8993(84)90246-4
Robert W. Stach, Barbara J. Wagner, Decrease in the number of lower affinity (type II) nerve growth factor receptors on embryonic sensory neurons does not affect fiber outgrowth. Journal of Neuroscience Research. ,vol. 7, pp. 103- 110 ,(1982) , 10.1002/JNR.490070202
P. K. SIITERI, Receptor binding studies. Science. ,vol. 223, pp. 191- 193 ,(1984) , 10.1126/SCIENCE.223.4632.191-A
Kenneth H. Sonnenfeld, Douglas N. Ishii, Nerve growth factor effects and receptors in cultured human neuroblastoma cell lines Journal of Neuroscience Research. ,vol. 8, pp. 375- 391 ,(1982) , 10.1002/JNR.490080226
Edward J. Olender, Barbara J. Wagner, Robert W. Stach, Sequestration of125I-Labeled p Nerve Growth Factor by Embryonic Sensory Neurons Journal of Neurochemistry. ,vol. 37, pp. 436- 442 ,(1981) , 10.1111/J.1471-4159.1981.TB00474.X