Cytokine regulation of localized inflammation. Induction of activated B cells and IL-6-mediated polyclonal IgG and IgA synthesis in inflamed human gingiva.

摘要: It is well established that increased numbers of plasma cells occur in the localized tissues chronic inflammatory diseases such as adult periodontitis, and enzymatic isolation has shown most B lineage produce IgG-subclass with some IgA-subclass responses. would be importance to determine if excess production cytokines lesion account for these responses present study we have assessed gingival mononuclear cell (GMC) supernatants activate including IL-6R expression levels IL-6 present. Inasmuch limited (approximately 1 3 x 10(6) cells) GMC were obtained from surgically removed 400 mg), focused on analysis by this study. Further, initial evidence additional are produced induce resting been obtained. The PBMC individual patients cultured presence (or absence) Con A. Higher spontaneously when compared A-stimulated PBMC. When cultures supplemented same patient, high spot-forming (SFC), mainly IgG followed IgA isotype, seen. induction SFC was inhibited incubation a goat anti-human antibody. effect subclasses determined, response IgG1 greater than IgG2 IgG3 = IgG4 IgA1 IgA2, pattern remarkably similar distribution itself. To assess mediated activation, containing anti-IL-6 or purified 72 h. This treatment induced small proliferative elevated cells, but did not (G0/G1) enter cycle (S G2/M). Addition human rIL-6 day restored IgG- 7. Cytokine-induced also occurred vivo because freshly isolated expressed receptor.(ABSTRACT TRUNCATED AT WORDS)