Cytokine production by human milk cells and peripheral blood mononuclear cells from the same mothers.

摘要: Samples of milk (n = 80) and venous blood were collected at 5 weeks postpartum from 82 lactating mothers. Human cells peripheral mononuclear isolated the production interleukin-1β, interleukin-6, tumor necrosis factor-α in absence presence lipopolysaccharide was determined by enzyme-linked immunosorbent assay. spontaneously produced significantly less than stimulation. In vitro stimulation human with (500 ng/ml) for 24 hr increased cytokine approximately 40–50%, whereas responded to (200 up 350%. These observations suggest that are capable active involvement mammary gland have capacity respond further after leaving breast.