Antidiuretic effect of antimuscarinic agents in rat model depends on C-fibre afferent nerves in the bladder

摘要: What's known on the subject? and What does study add? Antichollnergic agents are anticipated to diminish storage symptoms, as well nocturia. Nevertheless, effect of this treatment polyuria related nocturia is not clear. By subgroup analysis data set from a phase III clinical trial antimuscarinic agent for OAB patients in Japan, imidafenacin was found improve with reduction nocturnal polyuria. This adds effects underlying mechanism decreasing urine production through inhibition C-fibre bladder water-leaded rats. Objective To evaluate mechanisms used decrease water-loaded rats. Subjects Methods Urine measured using cystostomy catheter female Sprague–Dawley rats every 2 h. The atropine, tolterodine investigated under conditions, which were induced by i.p. injection 15 mL saline. Blood samples collected determine levels antidiuretic hormone (ADH), aldosterone (ALD), atrial natriuretic peptide (ANP) brain (BNP) before, 2 8 h after, administration. To induce desensitization afferent nerves, resiniferatoxin (RTX)was injected s.c. or intravesically days before experiments. Results Urine increased reached its maximum 2 h after saline injection. Imidafenacin decreased rats, but ADH, ALD, ANP BNP different between imidafenacin-treated vehicle-treated rats. The inhibitory RTX-treated rats. Atropine did reduce production. Conclusion These results suggest that volume C-fibres bladder; thus, antimuscarinics could be beneficial polyuria.