Chromosome microdissection identifies cryptic sites of DNA sequence amplification in human ovarian carcinoma.

摘要: Abstract DNA sequence amplification contributes to the multistep process of carcinogenesis, and overexpression amplified genes has been shown contribute malignant phenotype. Cytogenetic analyses human tumor cells, including ovarian malignancies, frequently show cytological evidence in form double minutes homogeneously staining regions. In this report, we have combined techniques chromosome microdissection fluorescence situ hybridization (P. S. Meltzer et al. , Nat. Genet., 1: 24–28, 1992) identify composition chromosomal origin seven regions from cases cancer. Twelve specific band were identified as 11q, 12p, 16p, 19p, 19q. These results provide important insights into organization sequences within malignancies add further our recognition likely harbor development or progression