Effect of Zinc Upon Human and Murine Cell Viability and Differentiation

摘要: Most zinc studies show its benefits or changes that coincide with deficiency, but some have reported damages by supplements. In this work, the effects of in different cell lines (U-937, human monocytes, and murine bone marrow cells) were analyzed. The cells put their specific culture medium either alone a stimulant [1-phorbol 12-myristate 13-acetate (PMA) for U-937 granulocyte macrophage colony stimulating factor (GM-CSF) cells]. These preparations, without (0.05 to 1.0 mM), incubated microscopically analyzed on days 3, 9, 11. viability all cultivated 0.05 0.1 mM was similar controls (90%). With zinc, diminished (p < 0.005) 80% 50% monocytes cells; number increased three lines, there no differentiation. We conclude observed doses vary not only among species also according time exposed metal. same can stimulatory an inhibitory effect.