Development of a castrate resistant transplant tumor model of prostate cancer.

摘要: BACKGROUND Currently, limited mouse models that mimic the clinical course of castrate resistant prostate development currently exist. Such are urgently required to conduct pre-clinical studies assist in understanding disease progression and rational therapeutic strategies treat cancer. METHODS Wild type intact FVB male mice were injected by subcutaneous injection with Myc-CaP cells establish androgen sensitive tumors. Tumor bearing castrated resulting tumors serially passaged pre-castrated produce a bone fide tumor. RESULTS Immunohistochemical analysis revealed initial had strong nuclear transcriptional active receptor expression, as indicated marked c-MYC staining highly proliferative. Castration tumor animals resulted cytoplasmic relocation concurrent loss activity proliferation. Serial passaging refractory bona which pheno-copied original parental tumor. CONCLUSIONS Developing murine transplant model mimics human cancer will create better opportunities understand also allow for more rapid stratify novel therapies this lethal form cancer. Prostate 72:587–591, 2012. © 2011 Wiley Periodicals, Inc.