The in vitro and in vivo behaviour of retinal pigment epithelial cells cultured on ultrathin collagen membranes.

摘要: The transplantation of pigment epithelial cells as a therapeutic modality for retinal degeneration requires that the transplanted form monolayer in subretinal space will establish communication with photoreceptors. Since previous studies have shown suspension do not monolayer, it be necessary to transplant preformed cell monolayers at location exposed To monolayers, (RPE) were cultured on ultrathin collagen membranes. Cells examined morphology, characteristics differentiation and viability. Membrane degradation long-term biocompatibility vivo assessed following subconjunctival implantation rabbits. These RPE adhere, proliferate, acquire differentiated properties membrane has features similar Bruch's membrane. Membranes subconjunctivally subretinally exhibit excellent without any evidence inflammation or rejection. membranes those complete are amenable space. non-toxic elicit rejection inflammatory response when implanted