Sequence analysis and amplification by polymerase chain reaction of a cloned DNA fragment for identification of Mycobacterium tuberculosis.

摘要: Analysis of the 1,016-base-pair sequence a putative probe for identification Mycobacterium tuberculosis revealed two almost identical fragments 507 and 509 bases. From this pairs primers were synthesized (MtbAB MtbCD), ranging from 18 to 22 nucleotides, use in polymerase chain reactions (PCRs) with DNA six reference strains M. tuberculosis, as well type bovis, bovis BCG, kansasii, avium, intracellulare, scrofulaceum. Although there was amplification all mycobacterial included study, when used probes, predominant band, fragment CD H37Rv DNA, proved be more specific than original probe, pMTb4, was. Amplified little 1 fg (equivalent one-fifth an organism) could resolved on ethidium bromide-stained gels loaded 1/10 volume PCR. Furthermore, it possible amplify sequences frozen organisms which thawed prior Images