Advanced technologies for studies on protein interactomes.

摘要: One of the key challenges biology in post-genomic era is to assign function many genesrevealed by large-scale sequencing programmes, since only a small fraction gene can bedirectly inferred from coding sequence. Identifying interactions between proteins substantialpart understanding their function. The main technologies for investigating protein–protein interactionsand assigning functions include direct detection intermolecular through proteinmicroarray, yeast two-hybrid system, mass spectrometry fluorescent techniques visualize protein complexesor pull-down assays, as well detecting functional genes, such RNAiknock down or screening cDNA libraries. Over recent years, considerable advances have beenmade above techniques. In this review, we discuss some developments and impact on thegene annotation.