Effects of intramammary infusion of sage (Salvia officinalis) essential oil on milk somatic cell count, milk composition parameters and selected hematology and serum biochemical parameters in Awassi sheep with subclinical mastitis.

摘要: AIM The aims of this study were to evaluate the effects intramammary infusion sage (Salvia officinalis) essential oil (EO) on milk somatic cell count (SCC), composition parameters and selected hematology serum biochemical in 20 Awassi ewes affected with subclinical mastitis. MATERIALS AND METHODS dried leaves used extract EO by hydrodistillation. minimum inhibitory concentration (MIC) bactericidal (MBC) against Staphylococcus aureus determined broth dilution method. Ewes divided randomly into three main groups received one following treatments; Group 1 (n=5): Dimethyl sulfoxide (DMSO) alone (5 ml; 0.2 ml DMSO 4.8 saline), 2 Amoxicillin (3 ml), 3 (n=10): Sage solution [0.2 DMSO+1 EO+3.8 sterile saline]). All treatments administered each teat twice per day for consecutive days. Milk samples SCC components determination whole blood analyses collected before treatment (T0) at 24 (T24) 48 (T48) h after last treatment. RESULTS MIC MBC S. 12.5% 6.1%, respectively. was decreased significantly (p<0.05) T24 T48 amoxicillin treated groups. fat lactose increased while no significant changes observed percentages solids-not-fat, protein total solids. No any or observed. There local systemic side ewes. However, further clinical trials are warranted determine safety possible withdrawal times its recommendation use organic operations. CONCLUSION In study, mastitis resulted a decrease posttreatment. addition, animals that as well those antibiotic.