Corticotropin-releasing factor (CRF) is involved in the acute anorexic effect of α-melanocyte-stimulating hormone : A study using CRF-deficient mice

摘要: Abstract Alpha-melanocyte-stimulating hormone (α-MSH) and its receptors are critical indispensable for maintaining appropriate feeding behavior energy homeostasis in both mice humans. Corticotropin-releasing factor (CRF) is a candidate mediating the anorexic effect of α-MSH. In present study, we examined whether CRF involved α-MSH, using CRF-deficient (CRFKO) receptor antagonist. Intracerebroventricular administration NDP-MSH, synthetic α-MSH analogue, suppressed food intake wild-type (WT) mice. This was abolished by pretreatment with non-selective antagonist, astressin, suggesting that α-MSH-induced anorexia mediated receptor. CRFKO mice, NDP-MSH did not affect at an early phase (0–4 h). addition, mRNA levels hypothalamus were significantly increased NDP-MSH-treated Therefore, our findings, CRFKO, strongly support evidence acute On other hand, administered to led late (4–12 h), similar WT Further, similarly reduced during all types including WT, corticosterone replacement. The results would suggest suppression independent glucocorticoids CRF.