Presence of the cap-binding protein in initiation factor preparations from poliovirus-infected HeLa cells.

摘要: Crude preparations of initiation factors from mock-infected and poliovirus-infected HeLa cells were analyzed for the presence proteins which could be cross-linked to 5' cap group mRNA. A protein having an apparent molecular weight 26,000, similar cap-binding in rabbit reticulocytes described by Sonenberg Shatkin (Proc. Natl. Acad. Sci. U.S.A. 75:4843-4847, 1978), was found ribosomal salt wash both uninfected infected cells. Cross-linking this polypeptide inhibited analog m7GMP. In addition, cross-linking a approximate 60,000 similarly analog. The smaller fractionated 0 40% ammonium sulfate precipitate wash; larger 40 70% fraction. Although present wash, only able restore translation capped vesicular stomatitis virus mRNA extracts prepared