Relationship between Membrane Integrity and the Inhibition of Host Translation in Virus‐Infected Mammalian Cells

摘要: The inhibition of host protein synthesis after picornavirus infection, and its relationship to the modification membrane permeability has been studied. In encephalomyocarditis (EMC)-virus-infected L cells, cellular is reduced from third hour at which time an in 86Rb+ uptake by infected cell was observed. bulk viral takes place a profound ionic concentration occurred. This agreement with idea that optimal translation requires higher monovalent ions as compared synthesis. A parallel measurement (a) synthesis, (b) content, (c) potential (d) hygromycin B EMC-virus-infected poliovirus-infected HeLa indicates cells correlation exists between alterations synthesis. not apparent suggesting this system other factors, different ion alterations, are involved shut-off. However, system, proteins also when modified altered. The analysis shut-off lines EMC virus or poliovirus kinetic pattern variable depends on line used multiplicity infection each experiment. The ATP content picornavirus-infected examined throughout infection. A decrease nucleotide post-infection factor could perhaps contribute occurs late Another influence transport amino acids. For instance, glycine decreased beginning leakage methionine observed These modifications development cytopathic effect virus-infected cells.