Modification of phospholipase C and phospholipase A2 activities during poliovirus infection.

摘要: Abstract The infection of HeLa cells by poliovirus leads to profound alterations in the activities both phospholipase C and A23187-stimulated A2. As early as third hour after infection, activity is enhanced, measured increase inositol triphosphate (IP3) cells. By fifth post-infection there a 5-fold IP3 infected Therefore, synthesis bulk proteins genomes takes place containing high sustained IP3. This augmentation dependent on multiplicity used. Poliovirus gene expression required induce activity, since presence cycloheximide or guanidine blocked it. In contrast activation induced poliovirus, drastic blockade A23187-induced A2 release [3H]arachidonic acid medium. action because it was prevented 3-methylquercetin. To our knowledge this first report analyzing these two animal virus-infected findings described may help explain modifications membrane permeability lipid metabolism undergone poliovirus-infected