Genome-wide gene expression analysis for target genes to differentiate patients with intestinal tuberculosis and Crohn's disease and discriminative value of FOXP3 mRNA expression.

摘要: Background and aims: Crohn’s disease (CD) intestinal tuberculosis (ITB) are both chronic granulomatous conditions with similar phenotypic presentations. Hence, there is need for a biomarker to differentiate between these two diseases. This study aimed at genome-wide gene expression analysis of colonic biopsies from confirmed cases ITB CD in comparison controls. To evaluate the role T regulatory cells, forkhead box P3 (FOXP3) mRNA was quantified serum as well patients Methods: Paired samples, including biopsies, were taken 33 subjects (CD, controls), total RNA extracted. Human whole genome microarray performed using Illumina HumanWG-6 BeadChip Kit six samples three groups duplicates. Real-time PCR FOXP3 analyzed biopsy (4-CD, 5-ITB, 4-controls). Results: In 1.5-fold upregulation 92 382 genes downregulation 91 256 genes, respectively. Peroxisome proliferators via PPARc pathway most significantly downregulated (P 2-fold change. ITB, complement activation pathway, specifically classical upregulated. elevated obtained compared (4.70 6 2.21 vs 1.48 0.31, P ¼ 0.016). Conclusions: mucosa could be discriminatory marker CD. Upregulation suggests that pathogenetic mechanisms those pulmonary tuberculosis. CD, seen tissue, suggesting restoration PPARc-dependent anti-microbial barrier function may therapeutic target.