A multivariate approach based on physicochemical parameters and biological potential for the botanical and geographical discrimination of Brazilian bee pollen

作者: Adriane Alexandre Machado De-Melo , Leticia M. Estevinho , Manuela M. Moreira , Cristina Delerue-Matos , Alex da Silva de Freitas

DOI: 10.1016/J.FBIO.2018.08.001

关键词:

摘要: Abstract Fifty six Brazilian bee pollen samples were characterised, and their botanical geographical origins studied. The also classified based on physicochemical biological properties. characterisation was done using Soxhlet extraction micro-Kjeldahl methods, ashing at 550 °C, transmittance, microscopy, spectrophotometry, inductively coupled plasma optical emission spectrometry high-performance liquid chromatography. As expected, the values of proteins (7.9–32.2 g/100 g), lipids (3.2–13.5 g/100 g), ash (1.9–3.6 g/100 g), carbohydrates (54.9–82.8 g/100 g), K (3.4–9.8 g/kg), Ca (0.9–4.1 g/kg), Mg (0.6–2.4 g/kg), Fe (46–1180 mg/kg), Na (20–374 mg/kg), Mn (25–215 mg/kg), Zn (30–101 mg/kg), Cu (7.4–19.7 mg/kg), total phenolics (6.5–29.2 mg GAE/g) flavonoids (0.3–17.5 mg QE/g) as well phenolic profiles varied among samples. In general, products had high antioxidant capacity (ORAC: 133–563 μmol TE/g; DPPH: 9.4–155 μmol TE/g) antimicrobial according to sample microorganism tested. instrumental colour parameters could be used indicators some minerals. Based multivariate approaches, from Southern Brazil seemed have a higher potential, whereas Northeast region calcium magnesium levels. Similarities identified in containing C. nucifera or Brassica, monofloral mineral content than heterofloral pollen.

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