Genetic Variation, Not Cell Type of Origin, Underlies Regulatory Differences in iPSCs

摘要: The advent of induced pluripotent stem cells (iPSCs) revolutionized Human Genetics by allowing us to generate from easily accessible somatic tissues. This technology can have immense implications for regenerative medicine, but iPSCs also represent a paradigm shift in the study complex human phenotypes, including gene regulation and disease. Yet, an unresolved caveat iPSC model system is extent which reprogrammed retain residual phenotypes their precursor cells. To directly address this issue, we used effective design compare regulatory between derived two types commonly We show that cell type origin only minimally affects expression levels DNA methylation iPSCs. Instead, genetic variation main driver differences different donors.