Mutation scanning analysis of sequence heterogeneity in the second internal transcribed spacer (rDNA) within some members of the Hypodontus macropi (Nematoda: Strongyloidea) complex

摘要: Single-strand conformation polymorphism analysis was employed to investigate sequence variation in the second internal transcribed spacer (ITS-2) of nuclear ribosomal DNA within and among individuals representing three operational taxonomic units (OTUs) Hypodontus macropi from different species Australian macropodid marsupials. Of 96 nematodes analysed, totals 3 (OTU1 Petrogale persephone), 10 (OTU2 Macropus robustus) 7 (OTU9 rufus) representative were selected for sequencing characterise estimate magnitude nucleotide ITS-2. While no unequivocal difference ITS-2 detectable OTU1, most (3/44.7%) detected OTU2 OTU9 related chiefly dinucleotide (CA, TA, or a combination both) differences. This microsatellite variability some H. OTUs suggests that rDNA may be subjected slippage events during replication, resulting short repeat tracts being dispersed throughout lineages, possibly transposition and/or crossing-over events. Nucleotide individual proposed secondary structure precursor RNAs. Most heterogeneity occurred loops bulges predicted structure, which appear not under functional constraint. The findings this study have implications investigating speciation population differentiation at molecular level.