Photoregulated assembly/disassembly of DNA‐templated protein arrays using modified oligonucleotide carrying azobenzene side chains

摘要: DNA-templated self-assembly of nanomaterials provides great potential for applications including biosensors, nanoelectronics, and programmable autonomous molecular machines. To switch or regulate the activities those nanobiotechnological devices, non-invasive methods to assemble disassemble specific nanoscale components are needed. Here, we describe photocontrol assembly protein arrays in solution, by using photo-controlled duplex formation oligonucleotides carrying azobenzene. As a proof concept prototype, designed one-dimensional array system that consists scaffold DNA two kinds anchor were conjugated with fluorescent proteins (CFP YFP, respectively). The was modified carry multiple azobenzene side chains so hybridization involving is regulated photoirradiation through conformational changes moieties. Melting temperatures made an oligonucleotide shifted significantly reversibly UV visible (difference T(m) 34.8 degrees C 150 mM potassium acetate). Measurements Forster resonance energy transfer between CFP YFP showed also changed photoirradiation. Such reversible method control assembly/disassembly multiple, would provide many functions devices such as on/off switches ability change configuration reversibly.