Molecular Design for Reversing the Photoswitching Mode of Turning ON and OFF DNA Hybridization
作者： Xingguo Liang , Nobutaka Takenaka , Hidenori Nishioka , Hiroyuki Asanuma
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摘要: A new photoswitch for DNA hybridization involving para-substituted azobenzenes (such as isopropyl- or tert-butyl-substituted derivatives) with L-threoninol a linker was synthesized. Irradiation of the modified visible light led to dissociation duplex owing destabilization effect bulky substituent on trans-azobenzene. In contrast, trans-to-cis isomerization (UV irradiation) facilitated formation. The direction this photoswitching mode entirely reversed relative previous system an unmodified azobenzene D-threoninol whose trans form turned hybridization, and cis it off. Such reversible directly demonstrated by using fluorophore- quencher-attached oligonucleotides. Furthermore, revealed that cis-to-trans thermal greatly suppressed in presence complementary strand formation more-stable form.
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