A Versatile Platform for Single- and Multiple-Unnatural Amino Acid Mutagenesis in Escherichia coli

作者: Abhishek Chatterjee , Sophie B. Sun , Jennifer L. Furman , Han Xiao , Peter G. Schultz

DOI: 10.1021/BI4000244

关键词:

摘要: … up to three UAAs within the same protein at levels up to 30% of the level of wild-type protein … Finally, we demonstrated that pUltra can serve as a platform for the integration of two …

参考文章(26)
J. Christopher Anderson, Peter G. Schultz, Adaptation of an orthogonal archaeal leucyl-tRNA and synthetase pair for four-base, amber, and opal suppression. Biochemistry. ,vol. 42, pp. 9598- 9608 ,(2003) , 10.1021/BI034550W
Wenyuan Wang, Jeffrey K Takimoto, Gordon V Louie, Thomas J Baiga, Joseph P Noel, Kuo-Fen Lee, Paul A Slesinger, Lei Wang, Genetically encoding unnatural amino acids for cellular and neuronal studies Nature Neuroscience. ,vol. 10, pp. 1063- 1072 ,(2007) , 10.1038/NN1932
Douglas D. Young, Travis S. Young, Michael Jahnz, Insha Ahmad, Glen Spraggon, Peter G. Schultz, An Evolved Aminoacyl-tRNA Synthetase with Atypical Polysubstrate Specificity, Biochemistry. ,vol. 50, pp. 1894- 1900 ,(2011) , 10.1021/BI101929E
Trudy H Grossman, Ernest S Kawasaki, Sandhya R Punreddy, Marcia S Osburne, Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability Gene. ,vol. 209, pp. 95- 103 ,(1998) , 10.1016/S0378-1119(98)00020-1
Travis S. Young, Insha Ahmad, Jun A. Yin, Peter G. Schultz, An enhanced system for unnatural amino acid mutagenesis in E. coli. Journal of Molecular Biology. ,vol. 395, pp. 361- 374 ,(2010) , 10.1016/J.JMB.2009.10.030
Travis S. Young, Insha Ahmad, Ansgar Brock, Peter G. Schultz, Expanding the genetic repertoire of the methylotrophic yeast Pichia pastoris. Biochemistry. ,vol. 48, pp. 2643- 2653 ,(2009) , 10.1021/BI802178K
Heinz Neumann, Kaihang Wang, Lloyd Davis, Maria Garcia-Alai, Jason W. Chin, Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome Nature. ,vol. 464, pp. 441- 444 ,(2010) , 10.1038/NATURE08817
A. Ambrogelly, S. Gundllapalli, S. Herring, C. Polycarpo, C. Frauer, D. Soll, Pyrrolysine is not hardwired for cotranslational insertion at UAG codons. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 104, pp. 3141- 3146 ,(2007) , 10.1073/PNAS.0611634104
S. E. Conrad, M. Wold, J. L. Campbell, Origin and direction of DNA replication of plasmid RSF1030. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 76, pp. 736- 740 ,(1979) , 10.1073/PNAS.76.2.736