Characterization of a new endo-type alginate lyase from Vibrio sp. W13.
作者： Benwei Zhu , Haidong Tan , Yuqi Qin , Qingsong Xu , Yuguang Du
DOI: 10.1016/J.IJBIOMAC.2015.01.053
关键词:
摘要: A gene, encoding a new alginate lyase Algb, was identified and cloned from marine bacterium Vibrio sp. W13. The recombinant characterized followed by being purified on Ni-NTA Sepharose. It exhibited the highest activity (457 U/mg) at pH 8.0 30 degrees C Interestingly, Algb possessed broader substrate specificity. showed activities toward both polyM (poly beta-D-mannuronate) polyG alpha-L-guluronate). Furthermore, K-m values of (0.67 mg/ml) polyMG (0.50 are lower than those (1.04 (6.90 mg/ml). TLC ESI-MS analysis suggested that mainly released oligosaccharides with DP 2-5 four kinds substrates in an endolytic manner. Therefore, it may be potent tool to produce low DP. (C) 2015 Elsevier B.V. All rights reserved.
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